Cryo-electron microscopy structure of yeast Ty retrotransposon virus-like particles.
نویسندگان
چکیده
The virus-like particles (VLPs) produced by the yeast retrotransposon Ty1 are functionally related to retroviral cores. These particles are unusual in that they have variable radif. A paired mass-radius analysis of VLPs by scanning transmission electron microscopy showed that many of these particles form an icosahedral T-number series. Three-dimensional reconstruction to 38-A resolution from cryo-electron micrographs of T = 3 and T = 4 shells revealed that the single structural protein encoded by the TYA gene assembles into spiky shells from trimeric units.
منابع مشابه
Symmetry, flexibility and permeability in the structure of yeast retrotransposon virus-like particles.
The virus-like particles (VLPs) of the yeast retrotransposon Ty are genetically, structurally and functionally analogous to retroviral nucleocapsids or cores. Like retroviral cores Ty-VLPs package and possibly promote the enzyme activities for reverse transcription and integration, as well as encapsulating the RNA that is the intermediate in retrotransposition. Here we show that Ty-VLPs assembl...
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عنوان ژورنال:
- Journal of virology
دوره 71 9 شماره
صفحات -
تاریخ انتشار 1997